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PubMed · 10800777

Structural elements determining activation kinetics in Kv2.1.

A Scholle·R Koopmann·T Leicher·J Ludwig·O Pongs·K Benndorf
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Abstract

Voltage-dependent K+ channels open when depolarizing the membrane voltage. Among the different alpha-subunits, the time course of current activation spreads over a wide range. The structural basis underlying this diversity is not known. We constructed multiple chimeras between two voltage-dependent K+ channels, the rapidly activating Kv1.2 and the slowly activating Kv2.1, and we focused on the C-terminal half of the core region. The general strategy was to substitute parts of Kv2.1 by corresponding parts of Kv1.2 and to test for an acceleration of activation. We identified three regions which contribute to the determination of the activation kinetics: the S5-pore linker, the deep pore, and the S4-segment.

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Amino Acid SequenceAnimalsDelayed Rectifier Potassium ChannelsHumansKineticsMolecular Sequence DataMutagenesisPotassium ChannelsPotassium Channels, Voltage-GatedRatsRecombinant Fusion ProteinsSequence Homology, Amino AcidShab Potassium ChannelsStructure-Activity RelationshipXenopus laevisJournal Article

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A Scholle, R Koopmann, T Leicher, J Ludwig, O Pongs, K Benndorf. 2000. Structural elements determining activation kinetics in Kv2.1.. https://pubmed.ncbi.nlm.nih.gov/10800777/

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