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PubMed · 10805129

Sensitivity enhancement in (HCA)CONH experiments.

R Folmer·G Otting
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Abstract

A novel sensitivity-enhancement technique is proposed for experiments which correlate protein backbone resonances and start with magnetization from 13Calpha-1Halpha groups. The technique is based on replenishing magnetization lost by dipole-CSA cross-correlated relaxation of the 13Calpha spin with 13Calpha steady state magnetization. The principle is demonstrated for the (HCA)CONH experiment, resulting in 1.6-fold sensitivity enhancement compared to the HN(CA)CO experiment. Furthermore, other versions of the (HCA)CONH experiment were evaluated, including a novel experiment with spin-locking of transverse 13C-1H two-spin coherence, and a cross-correlation compensated (CA)CONH experiment which starts from 13C rather than 1H magnetization.

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Bacterial ProteinsCarrier ProteinsDesulfovibrio vulgarisFlavoproteinsHydrogen-Ion ConcentrationNuclear Magnetic Resonance, BiomolecularUbiquitinsJournal ArticleResearch Support, Non-U.S. Gov't

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R Folmer, G Otting. 2000. Sensitivity enhancement in (HCA)CONH experiments.. https://doi.org/10.1023/a%3A1008308106108

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