Phenylalanyl-tRNA synthetase interacts with DNA: studies on activity using deoxyribooligonucleotides.

K A Ivanov; N A Moor; V N Ankilova; O I Lavrik

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PubMed · 10810180

Phenylalanyl-tRNA synthetase interacts with DNA: studies on activity using deoxyribooligonucleotides.

K A Ivanov·N A Moor·V N Ankilova·O I Lavrik

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Abstract

Phenylalanyl-tRNA synthetase from Thermus thermophilus complexes with short (up to 30 nucleotide length) single-stranded DNA fragments more efficiently than with double-stranded fragments. The complexing between DNA and the protein significantly increases with deoxyribooligonucleotide longer than 20 nucleotides. Using affinity labeling, the binding site of DNA was located near the interface of the alpha- and beta-subunits. The binding sites of DNA and tRNAPhe do not overlap. Phenylalanyl-tRNA synthetase from E. coli also binds DNA.

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DNA DNA, Single-Stranded Deoxyribonucleotides Electrophoresis, Polyacrylamide Gel Hydrogen-Ion Concentration Oligonucleotides Phenylalanine-tRNA Ligase Photoaffinity Labels RNA, Transfer Thermus thermophilus Transcription, Genetic Journal Article Research Support, Non-U.S. Gov't

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K A Ivanov, N A Moor, V N Ankilova, O I Lavrik. 2000. Phenylalanyl-tRNA synthetase interacts with DNA: studies on activity using deoxyribooligonucleotides.. https://pubmed.ncbi.nlm.nih.gov/10810180/

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