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PubMed · 10814975

Developmental changes in capacitative Ca(2+) entry in mouse mammary epithelial cells.

J Ichikawa·K Furuya·S Miyata·T Nakashima·T Kiyohara
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Abstract

Developmental changes in capacitative Ca(2+) entry and Ca(2+) release from intracellular stores were measured using fura-2 fluorescence method during the pregnancy period (day 3-;18) in mouse mammary epithelial cells. Ca(2+) release was identified with the transient intracellular Ca(2+) ([Ca(2+)](i)) increase induced by thapsigargin addition in a Ca(2+)-free solution. Capacitative Ca(2+) entry was measured by the transient [Ca(2+)](i) increase induced by re-addition of extracellular Ca(2+) after depletion of Ca(2+) stores by thapsigargin. The capacitative Ca(2+) entry was greatest at the early stage of pregnancy (i.e. day 3 of pregnancy) and decreased as pregnancy progressed, while Ca(2+) release remained unchanged throughout the developmental stages. These findings indicate that in contrast to Ca(2+) release, a close correlation exists between capacitative Ca(2+) entry and pregnancy-induced development in mammary epithelial cells.

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AnimalsCalciumCalcium-Transporting ATPasesCells, CulturedEnzyme InhibitorsEpitheliumFemaleFura-2Mammary Glands, AnimalMiceMice, Inbred ICRPregnancyPregnancy, AnimalSpectrometry, FluorescenceThapsigarginTime FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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J Ichikawa, K Furuya, S Miyata, T Nakashima, T Kiyohara. 2000. Developmental changes in capacitative Ca(2+) entry in mouse mammary epithelial cells.. https://doi.org/10.1002/(sici)1099-0844(200006)18%3A2%3C147%3A%3Aaid-cbf864%3E3.0.co%3B2-w

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