PubMed Health⌕ Search

PubMed · 10817647

'Infectious web'.

Abstract

The current web summary highlights internet sites describing the growing problem of Staphylococcus aureus antibiotic resistance, the epidemiology and prevention of malaria, biochemical and genetic aspects of lipopolysaccharide action, and the history and treatment of hepatitis C infections.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

2000. 'Infectious web'.. https://pubmed.ncbi.nlm.nih.gov/10817647/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

Dual-species interactions with intestinal bacteria drive multi-drug resistance in Campylobacter.

OBJECTIVES: Multidrug-resistant (MDR) Campylobacter infections are an increasing clinical concern, as rising fluoroquinolone (FQ) resistance leaves macrolides as the primary treatment option. We investigated multidrug resistance in clinical Campylobacter samples from Germany. METHODS: We analyzed 6980 clinical isolates (2010-2022), performing phenotypic susceptibility testing and sequencing on 2912 genomes. Cultures showing multidrug resistance were studied using scanning electron microscopy (SEM). RESULTS: We found that 453 (6%) Campylobacter samples were resistant to both FQ and macrolides. Two of the C. jejuni samples were resistant to antibiotics from ten different classes. Genome analysis revealed that these samples, despite being derived from single colonies, contained >10% Enterococcus DNA reads. SEM confirmed the presence of coccoid bacteria interspersed with spiral-shaped Campylobacter. Additional culture-based purification resulted in pure C. jejuni isolates that retained FQR but lost macrolide resistance. The presence of MDR Enterococcus spp. in the mixed samples protected C. jejuni from above-MIC (minimum inhibitory concentration) concentrations of several ribosome-targeting antimicrobials whereas pure Campylobacter were susceptible. CONCLUSIONS: The impact of microbial interactions on resistance phenotypes is poorly understood. We show that close interactions with highly resistant intestinal bacteria can induce multidrug resistance phenotypes in Campylobacter. These findings highlight that microbial context shapes antibiotic resistance and may influence treatment outcomes.

Campylobacter jejuni↗

Campylobacter-stimulated INT407 cells produce dissociated cytokine profiles.

OBJECTIVES: To study the action of factors produced by living Campylobacter jejuni (C. jejuni) against those present within sonicated and filtrated bacteria on induction of potential cytokines by the human intestinal cell line INT407. METHODS: We used immunohistochemical technique modified to detect intracellular production of cytokines protein and RT-PCR to read RNA messages for evaluation of de novo cytokine synthesis. RESULTS: The data herein display dissociation of cytokine profiles induced on by living C. jejuni. Exposure of INT407 cells to 10(6) live bacteria showed the highest numbers of cytokine producing cells of all examined cytokines. IFN-gamma was the highest induced cytokine followed by IL-10, TNF-alpha and lastly IL-4. Also, abrogation of induction of the proinflammatory cytokines IFN-gamma and TNF-alpha but not the antiinflammatory cytokines IL-4 and IL-10 by sonicated and filtrated bacteria was depicted. At the mRNA level, TNF-alpha signals were noted in accordance with its protein levels since increased TNF-alpha mRNA signals were registered only after stimulation with living bacteria. Very low or no induction of TNF-alpha was registered with non-stimulated cells. CONCLUSIONS: These results illustrate for the first time a role for factors from living bacteria in directing the immune response towards Th1 type. Characterization of such factors may be essential for future immunotherapeutic interventions during severe bacterial infections.

Campylobacter jejuni↗

Description of a "phoenix" phenomenon in the growth of Campylobacter jejuni at temperatures close to the minimum for growth.

When Campylobacter jejuni cultures that had been grown in broth at 39 degrees C were subcultured into fresh medium at 30 degrees C, there was a transient period of growth followed by a decline in viable-cell numbers before growth resumed once more. We propose that this complex behavior is the net effect of the growth of inoculum cells followed by a loss of viability due to oxidative stress and the subsequent emergence of a spontaneously arising mutant population that takes over the culture.

Campylobacter jejuni↗