Müller cell function during spreading depression in frog retina.

S Mori; W H Miller; T Tomita

doi:10.1073/pnas.73.4.1351

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Müller cell function during spreading depression in frog retina.

S Mori·W H Miller·T Tomita

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Abstract

K+ potentials were investigated using K+ microelectrodes in frog (Rana pipiens) retinas conditioned for spreading depression (SD) by Cl--free Ringer's solution. A marked increase in outside K+ concentration, [K+]O, maximal in the inner plexiform layer, was observed during SD. This [K+]o change resembled the simultaneously recorded membrane potential change in Müller cells, suggesting that these cells act as K+ electrodes. Intracellular recording with K+ electrodes in Müller cells showed that upon SD the [K+]i (inside) of Müller cells increases and therefore immediately starts to cleanse the extracellular space of the excess of K+ which, evidence suggests, is mainly caused by pathologically enhanced synaptic activity in the inner plexiform layer.

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Animals In Vitro Techniques Membrane Potentials Models, Biological Neuroglia Potassium Rana pipiens Retina Journal Article Research Support, U.S. Gov't, P.H.S.

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S Mori, W H Miller, T Tomita. 1976. Müller cell function during spreading depression in frog retina.. https://doi.org/10.1073/pnas.73.4.1351

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