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PubMed · 10853670

New fluorogenic substrate for the first continuous steroid sulfatase assay.

M Bilban·A Billich·M Auer·P Nussbaumer
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Abstract

The screening for new inhibitors of steroid sulfatase requires an efficient test system. To overcome the shortcomings of the available discontinuous fluorimetric assay, several coumarin-type compounds were investigated as potential new substrates. 3,4-Benzocoumarin 7-O-sulfate was found to have appropriate substrate properties for the establishment of the first direct continuous assay of steroid sulfatase.

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ArylsulfatasesChemical PhenomenaChemistry, PhysicalCoumarinsEnzyme InhibitorsFluorescent DyesFluorometryHumansKineticsRecombinant ProteinsSteryl-SulfataseStructure-Activity RelationshipSulfatasesJournal Article

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M Bilban, A Billich, M Auer, P Nussbaumer. 2000-05-01. New fluorogenic substrate for the first continuous steroid sulfatase assay.. https://doi.org/10.1016/s0960-894x(00)00144-x

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