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PubMed · 10869664

Progress in packed column supercritical fluid chromatography: materials and methods.

C F Poole
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Abstract

This article summarizes recent developments in packed column supercritical fluid chromatography. Silica-based chemically bonded sorbents, similar to those used for HPLC, are widely used with solvent-modified fluids containing additives to suppress undesirable solute-sorbent interactions that lead to poor peak shapes. Composition programming is the most useful approach to gradient elution separations since solvent-modified fluids have low compressibility. Packed column SFC is most useful for the separation of mixtures usually separated by normal-phase HPLC. Compared to normal-phase HPLC it offers faster separations, higher efficiencies, faster column re-equilibration, and a wider range of experimental variables for optimization. Packed column SFC is being increasingly selected for the analytical and preparative separation of racemic mixtures using enantiomer-selective sorbents.

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C F Poole. 2000-07-05. Progress in packed column supercritical fluid chromatography: materials and methods.. https://doi.org/10.1016/s0165-022x(00)00057-9

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