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PubMed · 10872420

Mechanisms underlying regulated CFTR trafficking.

K W Peters·J Qi·S C Watkins·R A Frizzell
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Abstract

Stimulation of membrane capacitance and cell surface labeling of epitope-tagged CFTR provide evidence of cAMP-regulated CFTR trafficking. Co-expression of syntaxin 1A inhibits cAMP-stimulated current and capacitance changes in CFTR expressing cells and blocks cAMP-induced increases in cell surface CFTR. Inhibition of CFTR trafficking by syntaxin over-expression suggests a role for SNARE proteins in this process. CFTR phosphorylation may alter physical interactions with SNARE proteins to regulate plasma membrane CFTR density.

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Cyclic AMPCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorGene Expression RegulationHumansMembrane ProteinsQa-SNARE ProteinsSyntaxin 1Journal ArticleReview

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K W Peters, J Qi, S C Watkins, R A Frizzell. 2000. Mechanisms underlying regulated CFTR trafficking.. https://doi.org/10.1016/s0025-7125(05)70246-7

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