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PubMed · 10880109

Peptoid-containing collagen mimetics with cell binding activity.

G Johnson·M Jenkins·K M McLean·H J Griesser·J Kwak·M Goodman·J G Steele
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Abstract

Collagen mimetic peptides containing the peptoid residue Nleu (Goodman Bhumralkar, Jefferson, Kwak, Locardi. Biopolymers 1998;47:127-142) were tested for interactions with epithelial cells and fibroblasts. Molecules containing the sequence Gly-Pro-Nleu with a minimum of nine repeats showed cell binding activity. The activity of these molecules appeared to be conformationally sensitive, with the triple-helical form being preferred. When immobilized on a surface, the (Gly-Pro-Nleu)(10)-Gly-Pro-NH(2) sequence stimulated the attachment and growth of corneal epithelial cells and fibroblasts and the migration of epithelial tissue. The peptide sequence KDGEA inhibited cell attachment to the (Gly-Pro-Nleu)(10)-Gly-Pro-NH(2) sequence, suggesting that cell binding to this collagen mimetic involves the alpha2beta1 heterodimer integrin receptor. Interestingly, peptides containing the sequence (GlyNleu-Pro-)(10)-NH(2) did not have cell binding activity. The discovery that triple-helical peptides containing the Gly-Pro-Nleu sequences interact with cells opens up new opportunities in the design of collagen mimetic biomaterials.

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Amino Acid SequenceAnimalsBiocompatible MaterialsCattleCell AdhesionCell DivisionCells, CulturedCollagenCorneaDrug StabilityEpithelial CellsFibroblastsMaterials TestingOligopeptidesPeptoidsProtein DenaturationJournal ArticleResearch Support, Non-U.S. Gov't

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G Johnson, M Jenkins, K M McLean, H J Griesser, J Kwak, M Goodman, J G Steele. 2000-09-15. Peptoid-containing collagen mimetics with cell binding activity.. https://doi.org/10.1002/1097-4636(20000915)51%3A4%3C612%3A%3Aaid-jbm9%3E3.0.co%3B2-z

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