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PubMed · 10908345

Silanized nucleic acids: a general platform for DNA immobilization.

A Kumar·O Larsson·D Parodi·Z Liang
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Abstract

We have developed a method for simultaneous deposition and covalent cross-linking of oligonucleotide or PCR products on unmodified glass surfaces. By covalently conjugating an active silyl moiety onto oligonucleotides or cDNA in solutions we have generated a new class of modified nucleic acids, namely silanized nucleic acids. Such silanized molecules can be immobilized instantly onto glass surfaces after manual or automated deposition. This method provides a simple and rapid, yet very efficient, solution to the immobilization of prefabricated oligonucleotides and DNA for chip production.

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Cross-Linking ReagentsDNA, ComplementaryGlassNucleic Acid HybridizationOligodeoxyribonucleotidesOligonucleotide Array Sequence AnalysisOrganosilicon CompoundsPolymerase Chain ReactionSilanesJournal ArticleResearch Support, Non-U.S. Gov't

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A Kumar, O Larsson, D Parodi, Z Liang. 2000-07-15. Silanized nucleic acids: a general platform for DNA immobilization.. https://doi.org/10.1093/nar%2F28.14.e71

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