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PubMed · 10908353

Universal bases for hybridization, replication and chain termination.

M Berger·Y Wu·A K Ogawa·D L McMinn·P G Schultz·F E Romesberg
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Abstract

Several unnatural, predominantly hydrophobic nucleobases that pack efficiently in duplex DNA without hydrogen bonding functionalities are reported to circumvent the hydrogen bonding-based specificity, both during oligonucleotide hybridization and enzymatic DNA synthesis. The reported nucleoside analogs are efficient 'universal bases' for hybridization, template directed DNA synthesis and chain termination. Moreover, several of the universal bases function in their biological role, hybridization or replication, with an efficiency not significantly reduced relative to their natural counterparts.

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Base PairingDNADNA ReplicationDNA-Directed DNA PolymeraseDeoxyadenine NucleotidesDeoxycytosine NucleotidesDrug StabilityHot TemperatureHydrogen BondingIsoquinolinesMethylationNucleic Acid HybridizationNucleosidesOligonucleotidesSubstrate SpecificityTemplates, GeneticThymine NucleotidesJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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M Berger, Y Wu, A K Ogawa, D L McMinn, P G Schultz, F E Romesberg. 2000-08-01. Universal bases for hybridization, replication and chain termination.. https://doi.org/10.1093/nar%2F28.15.2911

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