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PubMed · 10923494

[Human bone morphogenetic protein-2: recombinant expression in E. coli].

Q Ma·G Dang·D Ma
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Abstract

OBJECTIVE: To explore the way of producing human bone morphogenetic protein-2(hBMP-2) for bone healing by using the gene engeneering techniques. METHODS: hBMP-2 cDNA fragment, which consists of 3' end partial propeptide and mature peptide sequence, was inserted into the multiple cloning site of expression vector pkpL-3a via ligation. The recombinant plasmid pkpL-3a/hBMP-2 was transformed into E. coli pOp2136. By the method of restriction map, the positive expression clone was selected. SDS-PAGE analysis showed a new foreign protein band near 27,000 after induction. The yield of induced hBMP-2 accouned for 10% of the total bacterial proteins. RESULTS: The partial purified recombinant hBMP-2 was implanted into Wistar rat thigh. After 4 weeks, histological analysis showed that it induced the proliferation of mesenchymal type cells and formation of new cartilage and bone in the implantation area. CONCLUSION: The hBMP-2 produced by gene engeneering techniques has the biologic capacity of ectopic bone formation.

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AnimalsBone Morphogenetic Protein 2Bone Morphogenetic ProteinsEscherichia coliFemaleGrowth SubstancesHumansMaleOsteogenesisProtein EngineeringRatsRats, WistarRecombinant ProteinsTransforming Growth Factor betaJournal Article

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Q Ma, G Dang, D Ma. 1998. [Human bone morphogenetic protein-2: recombinant expression in E. coli].. https://pubmed.ncbi.nlm.nih.gov/10923494/

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