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PubMed · 10926276

Specimen collection and handling.

Abstract

Pre-analytical factors should be known and standardized to obtain reliable laboratory test result. If they could not be standardized they should be recognized. The specimen should be representative, properly collected, preserved, and labeled. It is also important to prevent any infection to the patients, the phlebotomists and the environment. The ideal is to get the right sample for the right test at the right time.

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BibTeXRIS

M Suryaatmadja. 1999. Specimen collection and handling.. https://pubmed.ncbi.nlm.nih.gov/10926276/

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Therapeutic drug monitoring of tacrolimus with the dried blood spot method.

In a preliminary investigation an assay for tacrolimus based on fingerprick sampling and consecutive application as a blood spot on sampling paper has been developed. The dried blood spot was analysed by HPLC-tandem mass spectrometry. The validated range was 1-30 microg/l. Intra- and inter-assay variability for precision and accuracy was <7.5% and 15%, respectively. Tacrolimus concentrations of 24 stable out patients were compared after both blood spot sampling and conventional venous sampling. Method agreement was investigated with the methods of Passing and Bablok and Bland Altman and proved suitable for clinical use. The dried blood spot method for tacrolimus seems promising for patient monitoring.

Blood Specimen Collection↗