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Structural analysis of (methyl-esterified) oligogalacturonides using post-source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

G J van Alebeek·O Zabotina·G Beldman·H A Schols·A G Voragen
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Abstract

The use of post-source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the structural analysis of ((partly) methyl-esterified) oligogalacturonides (oligoGalA) is described. The fragmentation behavior of purified (un)saturated oligoGalA (degree of polymerization 3-6), methyl-esterified and methyl-glycosydated oligoGalA was studied. General fragmentation patterns are described and used for the elucidation of the positions of methyl esters on partly methyl-esterified oligoGalA. This technique now permits the determination of the position of methyl esters or other substituents on pectic fragments, helping in understanding the mode of action of pectinolytic enzymes.

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Carbohydrate ConformationCarbohydrate SequenceEstersMolecular Sequence DataOligosaccharidesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationJournal ArticleResearch Support, Non-U.S. Gov't

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G J van Alebeek, O Zabotina, G Beldman, H A Schols, A G Voragen. 2000. Structural analysis of (methyl-esterified) oligogalacturonides using post-source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.. https://doi.org/10.1002/1096-9888(200007)35%3A7%3C831%3A%3Aaid-jms7%3E3.0.co%3B2-4

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