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PubMed · 1095654

The macrophage electrophoretic mobility test (MEM) some technical considerations.

B K Shenton·E J Field
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Abstract

The following parameters of the cytopherometric test system have been studied. 1. Temperature is less important for the production of macrophage slowing factor (MSF) by lymphocyte-antigen interaction than it is for the activity of MSF on macrophages. 2. Lymphocyte number titration curves allow discrimination between 'weak' and 'strong' antigen responses. 3. Lymphocyte-antigen reaction reaches a plateau after 30 min incubation, whilst maximum MSF-macrophage reaction requires 60 min. 4. Interaction of MSF with macrophages is sensitive to cycloheximide. 5. Irradiation of peritoneal exudate cells (200 rad) is essential in order to obtain a maximal result in the MEM test. In the 'split MEM test, 200 rad to the indicator peritoneal exudate cells produces a greater result, i.e. it in some way amplifies the macrophage response to MSF. 6. Excess of peritoneal exudate cells, especially when non-irradiated, suppresses the test result. This is not due to consumption of MSF produced. Probably macrophages rather than lymphocytes are responsible for the suppression.

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AnimalsAscitic FluidCell Migration InhibitionCycloheximideGuinea PigsHumansLymphocytesMacrophage Migration-Inhibitory FactorsMacrophagesRadiation EffectsTemperatureTuberculinJournal Article

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B K Shenton, E J Field. 1975. The macrophage electrophoretic mobility test (MEM) some technical considerations.. https://doi.org/10.1016/0022-1759(75)90012-5

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