A broad-host-range plasmid for isolating mobile genetic elements in gram-negative bacteria.

D Schneider; D Faure; M Noirclerc-Savoye; A C Barrière; E Coursange; M Blot

doi:10.1006/plas.2000.1483

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A broad-host-range plasmid for isolating mobile genetic elements in gram-negative bacteria.

D Schneider·D Faure·M Noirclerc-Savoye·A C Barrière·E Coursange·M Blot

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Abstract

Plasmid pGBG1 was constructed to isolate mobile genetic elements in a wide variety of gram-negative bacteria. The mutation target, carried on a broad-host-range vector, allows positive selection for tetracycline resistance. In tests using several gram-negative bacteria we could detect transposition events of either insertion sequences or transposons. A new insertion sequence (IS) element was identified in Ralstonia eutropha.

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Agrobacterium tumefaciens Azospirillum Burkholderia cepacia Conjugation, Genetic Cupriavidus necator DNA Transposable Elements DNA, Bacterial Escherichia coli Genetic Techniques Gram-Negative Bacteria Mutagenesis, Site-Directed Plasmids Restriction Mapping Retroelements Journal Article Research Support, Non-U.S. Gov't

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D Schneider, D Faure, M Noirclerc-Savoye, A C Barrière, E Coursange, M Blot. 2000. A broad-host-range plasmid for isolating mobile genetic elements in gram-negative bacteria.. https://doi.org/10.1006/plas.2000.1483

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