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Apoptosis in human term placenta. A morphological and gene expression study.

M P Thiet·V Suwanvanichkij·K Hasselblatt·J Yeh
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Abstract

The objective of this study was to examine placentas after delivery from normal, healthy patients at term gestation. The placentas were from elective cesarean sections (n = 10, prior to the onset of labor) and spontaneous vaginal delivery (n = 10, after labor). We found that deoxyribonucleic acid laddering was present in all placentas and consistent with the pattern found in tissues that undergo apoptosis. Paraffin-embedded sections of placental villi stained by the in situ terminal deoxynucleotidyl transferase mediated biotinylated dUTP nick end labeling method revealed positive apoptotic nuclei in the placental villi. Reverse-transcriptase polymerase chain reaction demonstrated expression of messenger RNA for testosterone-repressed prostate message 2 and B cell lymphoma/leukemia-2 in the placenta. Our data demonstrate that apoptosis occurs in human term placenta.

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ApoptosisCesarean SectionClusterinDNA FragmentationFemaleGestational AgeGlycoproteinsHumansIn Situ Nick-End LabelingLabor, ObstetricMolecular ChaperonesPlacentaPregnancyProto-Oncogene Proteins c-bcl-2RNA, MessengerReverse Transcriptase Polymerase Chain ReactionJournal Article

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M P Thiet, V Suwanvanichkij, K Hasselblatt, J Yeh. 2000. Apoptosis in human term placenta. A morphological and gene expression study.. https://doi.org/10.1159/000010288

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