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PubMed · 10965406

Correlation between apoptotic figure counting and the TUNEL technique.

V Vagunda·J Kalabis·M Vagundová
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Abstract

OBJECTIVE: To compare histologic detection of apoptotic (AP) figures and terminal transferase UTP nick end labeling (TUNEL) detection in colorectal carcinomas and lymphomas. STUDY DESIGN: We evaluated the percentage of AP cells by AP figure counting and by the TUNEL technique in formalin-paraffin material from 39 tumors--29 colorectal carcinomas and 10 non-Hodgkin's lymphomas. The Lucia G image analysis system (Laboratory Imaging, Prague, Czech Republic) was used for cellularity evaluation and AP counting. RESULTS: On average, 0.81 +/- 0.5% AP cells were detected by figure counting; 0.91 +/- 0.35% were identified by TUNEL. A statistically high correlation between these techniques was found using Pearson's correlation coefficient (r = .56, P < .001). TUNEL, although probably more sensitive, was difficult to standardize. CONCLUSION: AP figure counting seems to be the method of choice for routine work, particularly because of its cost effectiveness and reproducibility.

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ApoptosisCarcinomaCell NucleusColorectal NeoplasmsHumansIn Situ Nick-End LabelingLymphoma, Non-HodgkinComparative StudyEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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V Vagunda, J Kalabis, M Vagundová. 2000. Correlation between apoptotic figure counting and the TUNEL technique.. https://pubmed.ncbi.nlm.nih.gov/10965406/

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