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IS1675, a novel lactococcal insertion element, forms a transposon-like structure including the lacticin 481 lantibiotic operon.

A Dufour·A Rincé·P Uguen·J P Le Pennec
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Abstract

Two copies of IS1675, a novel lactococcal insertion element from the IS4 family, are present on a 70-kb plasmid, where they frame the lantibiotic lacticin 481 operon. The whole structure could be a composite transposon designated Tn5721. This study shows that the lacticin 481 operon does not include any regulatory gene and provides a new example of a transposon-associated bacteriocin determinant. We identified five other IS1675 copies not associated with the lacticin 481 operon. The conservation of IS1675 flanking sequences suggested a 24-bp target site.

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Amino Acid SequenceAnti-Bacterial AgentsBacterial ProteinsBacteriocinsBase SequenceDNA Transposable ElementsDNA, BacterialGenes, BacterialLactococcus lactisMolecular Sequence DataOperonSequence Analysis, DNAJournal Article

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A Dufour, A Rincé, P Uguen, J P Le Pennec. 2000. IS1675, a novel lactococcal insertion element, forms a transposon-like structure including the lacticin 481 lantibiotic operon.. https://doi.org/10.1128/jb.182.19.5600-5605.2000

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