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PubMed · 11005931

All solid-state GFP sensor.

Y Kostov·C R Albano·G Rao
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Abstract

An all-solid-state green fluorescent protein (GFP) sensor for GFP measurement was developed. It is immune to interference from ambient light and works with standard flow-through cuvettes. The sensor is practically insensitive to the scattered excitation light encountered in microbial suspensions. It has a range of 0.0002-1 g/L (7.4 x 10(-9) - 3.7 x 10(-5) M) with limit of detection 0.00019 g/L (7.0 x 10(-9) M). The sensor could be used with a UV or blue light emitting diode (LED) as a light source, depending on required sensitivity, selectivity, and background levels. Its very low cost makes it useful in a variety of applications. This article describes the construction and validation of the sensor both off- and on-line in fermentation processes.

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Biosensing TechniquesEscherichia coliFermentationGreen Fluorescent ProteinsLuminescent ProteinsReproducibility of ResultsSensitivity and SpecificityJournal ArticleResearch Support, Non-U.S. Gov't

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Y Kostov, C R Albano, G Rao. 2000-11-20. All solid-state GFP sensor.. https://doi.org/10.1002/1097-0290(20001120)70%3A4%3C473%3A%3Aaid-bit14%3E3.0.co%3B2-2

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