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HLA-B44 subtyping in the Catalan population using reference strand mediated conformation analysis. Implications for the selection of unrelated bone marrow donors.

D Gallardo·J I Aróstegui·M Rodríguez-Luaces·S Querol·J R González·J García-López·A Grañena
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Abstract

Cytotoxic T lymphocytes (CTLs) reactive against the disparity between HLA-B*4402 and HLA-B*4403 have been reported after unrelated donor bone marrow transplantation. These CTLs have been associated with acute graft-versus-host disease and graft rejection. This study describes the HLA-B44-subtyping in the Catalan population using reference-strand mediated conformation analysis. It has been performed on 297 unrelated HLA-B44+ cord blood units from the Barcelona Cord Blood Bank (Barcelona, Spain). We have found a predominance of HLA-B*4403 (66.04%) over HLA-B*4402 (33.02%), whereas the predominant HLA-B44 allele in Northern Europe and the United States is HLA-B*4402. This inverted proportion between HLA-B44 subtypes in Mediterranean populations compared with other Caucasian populations suggests that HLA-B44 subtyping should be performed when an HLA-B44+ unrelated donor marrow is identified.

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AllelesBone Marrow TransplantationFetal BloodHLA-B AntigensHLA-B44 AntigenHumansImmunophenotypingPolymorphism, Single-Stranded ConformationalSpainT-Lymphocytes, CytotoxicTissue DonorsWhite PeopleJournal ArticleResearch Support, Non-U.S. Gov't

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BibTeXRIS

D Gallardo, J I Aróstegui, M Rodríguez-Luaces, S Querol, J R González, J García-López, A Grañena. 2000. HLA-B44 subtyping in the Catalan population using reference strand mediated conformation analysis. Implications for the selection of unrelated bone marrow donors.. https://doi.org/10.1034/j.1399-0039.2000.560212.x

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