PubMed · 11028601
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S Katzman. 2000-10-01. Screening for STDs.. https://doi.org/10.1021/ac002946x
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BACKGROUND & OBJECTIVE: Some genes are related to the occurrence of tumor. p53 is one of these kinds of gene. Mdm2 and p14 can modulate the function of p53. However, the reports about the function of mdm2 and p14 in gastric tumor are rare. The aim of this study was to observe the change of oncogene mdm2 and suppressor cancer gene p14 in gastric cancer cell and to investigate the relationship of mdm2, p14 with gastric cancer. METHODS: A total of 55 gastric mucosal biopsy specimens were enrolled, including 10 of normal gastric mucosa and 45 gastric tumor, which were proved pathologically as adenoma, including 15 poorly, moderately, and well differentiated specimens, respectively. The mRNA expression of mdm2 and p14 in gastric cancer were identified by reverse transcription polymerase chain reaction (RT-PCR), and the relationship of mdm2, p14 with gastric cancer was analyzed. RESULTS: (1) There was significant difference of the expression positive ratio of mdm2 mRNA between gastric tumor and normal gastric tissues (73.3% vs. 40%) (P< 0.05) [93.3% for the well differentiated specimens, 80.0% for the moderately differentiated specimens, and 46.6% for the poorly differentiated specimens]. There was significant difference between any two groups of the three specimens (P< 0.05). (2) There was no significant difference of the expression positive ratio of p14 mRNA between gastric tumor and normal gastric tissues (60.0% vs. 50.0%) (P >0.05) [60.0% for the well differentiated specimens, 66.6% for the moderately differentiated specimens, and 53.5% for the poorly differentiated specimens]. There was no significant difference between any two groups of the three specimens (P >0.05). CONCLUSION: The mRNA expression of mdm2 is closely correlated with the progression of gastric cancer. The relationship between p14 and gastric tumor has not been tested in this study and further study will be needed.
Comparison of partially sequenced cereal genomes suggests a mosaic structure consisting of recombinationally active gene-rich islands that are separated by blocks of high-copy DNA. Annotation of the whole rice genome suggests that most, but not all, cereal genes are present within the rice genome and that the high number of reported genes in this genome is probably due to duplications. Within the cereals, macrocolinearity is conserved but, at the level of individual genes, microcolinearity is frequently disrupted. Preliminary evidence from limited comparative analysis of sequenced orthologous genomic segments suggests that local gene amplification and translocation within a plant genome may be linked in some cases.
A preclinical evaluation of a qualitative assay for the detection of hepatitis C virus (HCV) RNA by transcription-mediated amplification (TMA) was conducted according to the guidelines of the National Committee for Clinical Laboratory Standards and the U.S. Food and Drug Administration. Our results showed that this assay, HCV TMA, detected 95% of samples with HCV RNA concentrations of 5.3 IU/ml and 29 copies/ml. HCV TMA showed an overall specificity of 99.6% and was highly reproducible, detecting 99.3% of samples with HCV RNA concentrations of 50 copies/ml across seven different lots of reagents. Experiments with clinical samples showed that HCV TMA detected all HCV genotypes with similar efficiencies, detecting > or = 95% of samples at 50 HCV RNA copies/ml from patients infected with HCV genotypes 1a, 2b, 3a, 4a, 5a, and 6a. In experiments with RNA transcripts, HCV TMA detected > or = 96.6% of transcripts derived from HCV genotypes 1a, 1b, 2a, 2c, 3a, 4a, 5a, and 6a at 50 HCV RNA copies/ml. Detection of transcripts derived from HCV genotype 2b was slightly lower (88.4%) at 50 copies/ml but was 97.0% at 75 copies/ml. In addition, HCV TMA exhibited robust performance in detecting HCV RNA in samples subjected to various conditions commonly encountered in a clinical laboratory, including long-term storage, multiple freeze-thaw cycles, different collection tubes, and the presence of endogenous substances, commonly prescribed drugs, or other microorganisms and viruses. With its high sensitivity, specificity, reproducibility, and equivalent genotype reactivity, HCV TMA may provide an attractive alternative for routine qualitative HCV RNA testing in clinical laboratories.