Complementation of Myc-dependent cell proliferation by cDNA expression library screening.

M A Nikiforov; I Kotenko; O Petrenko; A Beavis; L Valenick; I Lemischka; M D Cole

doi:10.1038/sj.onc.1203880

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Complementation of Myc-dependent cell proliferation by cDNA expression library screening.

M A Nikiforov·I Kotenko·O Petrenko·A Beavis·L Valenick·I Lemischka·M D Cole

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Abstract

The targeted knockout of the c-myc gene from rat fibroblasts leads to a stable defect in cell proliferation. We used complex cDNA libraries expressed from retroviral vectors and an efficient sorting procedure to rapidly select for cDNAs that can restore the growth rate of c-myc deficient cells. All of the biologically active cDNAs contained either c-myc or N-myc, suggesting that no other cellular genes can effectively bypass the requirement for c-myc in fibroblast proliferation. This approach provides a powerful screening method for cell cycle changes in genetically defined systems.

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Animals Cell Division DNA, Complementary Fibroblasts Gene Library Gene Targeting Genes, cdc Genes, myc Genetic Complementation Test Humans Mice Polymerase Chain Reaction Proto-Oncogene Proteins c-myc Rats Selection, Genetic Journal Article Research Support, U.S. Gov't, P.H.S.

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M A Nikiforov, I Kotenko, O Petrenko, A Beavis, L Valenick, I Lemischka, M D Cole. 2000-10-05. Complementation of Myc-dependent cell proliferation by cDNA expression library screening.. https://doi.org/10.1038/sj.onc.1203880

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