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A broad-host-range expression vector series including a Ptac test plasmid and its application in the expression of the dod gene of Serratia marcescens (coding for ribulose-5-phosphate 3-epimerase) in Pseudomonas stutzeri.

S Graupner·W Wackernagel
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Abstract

A series of expression vectors for gram-negative bacteria was constructed which combine broad-host-range, inducible expression from the tac promoter and diverse antibiotic resistance determinants. The tac promoter activity and the repression by lacIq can be quantitated with a separate test plasmid in the strain to be studied. The dod gene of Serratia marcescens was expressed in Pseudomonas stutzeri and was shown to code for D-ribulose-5-phosphate 3-epimerase.

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Bacterial ProteinsBase SequenceCarbohydrate EpimerasesDNA PrimersDNA, BacterialDrosophila ProteinsGene ExpressionGenes, BacterialGenetic VectorsGenetics, MicrobialInsect ProteinsNIMA-Interacting Peptidylprolyl IsomerasePeptidylprolyl IsomerasePolymerase Chain ReactionPromoter Regions, GeneticPseudomonasSerratia marcescensTransformation, Bacterialbeta-GalactosidaseJournal ArticleResearch Support, Non-U.S. Gov'tReview

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S Graupner, W Wackernagel. 2000. A broad-host-range expression vector series including a Ptac test plasmid and its application in the expression of the dod gene of Serratia marcescens (coding for ribulose-5-phosphate 3-epimerase) in Pseudomonas stutzeri.. https://doi.org/10.1016/s1389-0344(00)00061-7

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