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Direct coupling between meiotic DNA replication and recombination initiation.

V Borde·A S Goldman·M Lichten
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Abstract

During meiosis in Saccharomyces cerevisiae, DNA replication occurs 1. 5 to 2 hours before recombination initiates by DNA double-strand break formation. We show that replication and recombination initiation are directly linked. Blocking meiotic replication prevented double-strand break formation in a replication-checkpoint-independent manner, and delaying replication of a chromosome segment specifically delayed break formation in that segment. Consequently, the time between replication and break formation was held constant in all regions. We suggest that double-strand break formation occurs as part of a process initiated by DNA replication, which thus determines when meiotic recombination initiates on a regional rather than a cell-wide basis.

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Chromosomes, FungalDNA RepairDNA ReplicationDNA, FungalGenes, FungalMeiosisMutationRecombination, GeneticSaccharomyces cerevisiaeJournal Article

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V Borde, A S Goldman, M Lichten. 2000-10-27. Direct coupling between meiotic DNA replication and recombination initiation.. https://doi.org/10.1126/science.290.5492.806

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