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PubMed · 11053848

Expression, crystallization and preliminary X-ray diffraction studies of recombinant Bacillus anthracis lethal factor.

L Bernardi·G Vitale·C Montecucco·A Musacchio
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Abstract

The lethal factor (LF) produced by Bacillus anthracis is a Zn(2+)-dependent endopeptidase which specifically cleaves the N-terminal tail of several MAP kinase kinases (MAPKKs). The recombinant expression, purification and crystallization of LF and of an inactive mutant consisting of a single amino-acid substitution in the conserved catalytic site are reported here. Both proteins crystallize in the cubic space group I432.

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Bacillus anthracisBacterial ToxinsCrystallizationCrystallography, X-RayProtein ConformationRecombinant ProteinsJournal Article

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L Bernardi, G Vitale, C Montecucco, A Musacchio. 2000. Expression, crystallization and preliminary X-ray diffraction studies of recombinant Bacillus anthracis lethal factor.. https://doi.org/10.1107/s0907444900010027

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