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PubMed · 11071251

Osteoclast differentiation factor in human osteosarcoma cell line.

K Kinpara·M Mogi·M Kuzushima·A Togari
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Abstract

A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for human osteoclast differentiation factor (ODF/RANKL/ OPGL/TRANCE) utilizing a polyclonal antibody that recognizes both human soluble ODF and mouse ODF in combination with a osteoclasogenesis inhibitory factor (OCIF/OPG) was developed. We can quantify the ODF level in not only human ODF (detection limit: 0.05 ng/ml), but also mouse ODF by virtue of cross-reactivity. Employing this assay system, we demonstrated that ODF is constitutively present as a membrane-bound form in both the human osteosarcoma cell lines, MG-63, HOS and SaOS-2, and the mouse osteoblastic cell line MC3T3-E1.

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AnimalsCarrier ProteinsCell DifferentiationCross ReactionsEnzyme-Linked Immunosorbent AssayGlycoproteinsHumansMembrane GlycoproteinsMiceOsteoblastsOsteoclastsOsteoprotegerinOsteosarcomaProtein BindingRANK LigandReceptor Activator of Nuclear Factor-kappa BReceptors, Cytoplasmic and NuclearReceptors, Tumor Necrosis FactorTumor Cells, CulturedJournal ArticleResearch Support, Non-U.S. Gov't

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K Kinpara, M Mogi, M Kuzushima, A Togari. 2000. Osteoclast differentiation factor in human osteosarcoma cell line.. https://doi.org/10.1080/01971520009349540

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