Maternal estrogen receptor-beta expression during mouse gestation.

C Y Spong; S K McCune; E M Sternberg; J A Gustafsson

doi:10.1111/j.8755-8920.2000.440410.x

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PubMed · 11076098

Maternal estrogen receptor-beta expression during mouse gestation.

C Y Spong·S K McCune·E M Sternberg·J A Gustafsson

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Abstract

PROBLEM: Although estrogen receptor (ER)-alpha has been well characterized, the recently identified novel ER-beta has not. In some tissues, there is overlap of the ERs, which allows for rescue in cases of deficiency; in other tissues, the ERs appear to have opposite effects. The objective of this study was to evaluate the expression of ER-beta during pregnancy. METHOD OF STUDY: Pregnant mouse uteri (embryonic days 6-14, 16, 18) were studied. ER-alpha and ER-beta oligonucleotide probes were end-labeled and in situ hybridization histochemistry was performed. RESULTS: ER-beta was strongly expressed in maternal ovaries; there was no other evidence of strong expression during gestation. ER-alpha was expressed in the uterus throughout gestation, with decreasing intensity as gestation progressed, and in maternal ovarian tissue. CONCLUSIONS: Differential expression of the two ERs was apparent during pregnancy, with ER-alpha playing a dominant role. This may have implications for selective drug treatment targeting estrogen receptors.

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Animals Base Sequence Estrogen Receptor beta Female Gene Expression In Situ Hybridization Mice Oligonucleotide Probes Pregnancy Pregnancy, Animal RNA, Messenger Receptors, Estrogen Tissue Distribution Uterus Journal Article

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C Y Spong, S K McCune, E M Sternberg, J A Gustafsson. 2000. Maternal estrogen receptor-beta expression during mouse gestation.. https://doi.org/10.1111/j.8755-8920.2000.440410.x

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