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Surface-enhanced Raman scattering: a structure-specific detection method for capillary electrophoresis.

L He·M J Natan·C D Keating
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Abstract

A new approach to detecting capillary electrophoresis (CE) eluent components by interfacing CE with a surface-enhanced Raman scattering (SERS) system is described. In this approach, CE-based separation of a mixture of trans-1,2-bis(4-pyridyl)ethylene and N,N-dimethyl-4-nitrosoaniline has been detected by SERS in a postcolumn geometry. The retention time obtained from SERS corresponds well with that from conventional UV-visible detection. Meanwhile, CE eluants are identified by their characteristic vibrational spectra, demonstrating the validity of SERS as a structure-specific detection method for CE. In addition, the ability to monitor SERS intensity changes at molecule-specific frequencies makes selective detection of individual analytes possible, even when separation is incomplete. Finally, CE-SERS is evaluated for separation of amino acids (tyrosine and tryptophan) and environmental pollutants (chlorophenol mixtures).

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Aniline CompoundsChlorophenolsElectrophoresis, CapillaryNitroso CompoundsPyridinesSilverSpectrophotometrySpectrum Analysis, RamanTryptophanTyrosineJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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L He, M J Natan, C D Keating. 2000-11-01. Surface-enhanced Raman scattering: a structure-specific detection method for capillary electrophoresis.. https://doi.org/10.1021/ac000583v

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