LldI, a plasmid-encoded type I restriction and modification system in Lactococcus lactis.

Y M Deng; C Q Liu; N W Dunn

doi:10.3109/10425170009033237

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LldI, a plasmid-encoded type I restriction and modification system in Lactococcus lactis.

Y M Deng·C Q Liu·N W Dunn

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Abstract

A plasmid-encoded type I restriction and modification (R-M) system, designated LldI, was identified in Lactococcus lactis biovar diacetylactis LD10-1. LldI consists of three genes encoding endonuclease, methylase and specificity subunits, respectively. RT-PCR analysis revealed that the three genes are co-transcribed as a polycistronic mRNA in L. lactis. The specificity subunit of LldI differs significantly in the target recognition domains from those of other type I R-M systems, suggesting that LldI confers a novel specificity in L. lactis.

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Amino Acid Sequence Base Sequence Chromosome Mapping Conserved Sequence DNA Restriction Enzymes Deoxyribonucleases, Type I Site-Specific Genes, Bacterial Lactococcus lactis Molecular Sequence Data Plasmids Protein Subunits RNA, Messenger Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment Sequence Homology, Amino Acid Site-Specific DNA-Methyltransferase (Adenine-Specific)Transcription, Genetic Comparative Study Journal Article

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Y M Deng, C Q Liu, N W Dunn. 2000. LldI, a plasmid-encoded type I restriction and modification system in Lactococcus lactis.. https://doi.org/10.3109/10425170009033237

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