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PubMed · 11105860

Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2'-dipyridyldisulfide.

Z Glatz·H Maslanová
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Abstract

A new method for specific determination of glutathione using micellar electrokinetic chromatography and on-column reaction with 2,2'-dipyridyldisulfide is described. 2,2'-Dipyridyldisulfide and a sample of glutathione are injected consecutively into the capillary as two discrete plugs separated with a short plug of background electrolyte. Due to the differences in the mobilities of the 2,2'-dipyridyldisulfide and glutathione, on-column mixing and reaction occur. Glutathione is in this reaction quantitatively transformed into a mixed disulfide concomitantly with formation of an equimolar amount of the 2-thiopyridone which is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of glutathione is thus estimated indirectly from the result of 2-thiopyridone determination.

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2,2'-DipyridylChromatography, Micellar Electrokinetic CapillaryDisulfidesGlutathioneHumansSpectrophotometry, UltravioletJournal ArticleResearch Support, Non-U.S. Gov't

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Z Glatz, H Maslanová. 2000-10-20. Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2'-dipyridyldisulfide.. https://doi.org/10.1016/s0021-9673(00)00475-1

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