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PubMed · 11107184

Chromosome arm-specific multicolor FISH.

R Karhu·M Ahlstedt-Soini·M Bittner·P Meltzer·J M Trent·J J Isola
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Abstract

Several systems for 24-color fluorescence in situ hybridization (FISH) have been developed and applied to karyotyping and detection of chromosomal abnormalities. We have developed a 42-color multicolor FISH (mFISH) technique (armFISH), which permits the detection of chromosomal aberrations at the resolution of chromosome arms. The armFISH uses a commercially available mFISH reagent kit (24XCyte, MetaSystems GmbH) supplemented with a set of differentially labeled chromosome arm-specific painting probes (arm-kit, comprising either p- or q-arms of all human chromosomes, except the p-arm of the acrocentric- and Y chromosomes). The mFISH-probe cocktail and the arm-kit are combined and hybridized together to metaphase chromosomes. The armFISH is analyzed in two steps; first, the conventional mFISH image analysis is performed, followed by the arm-kit analysis to reveal the chromosome arms involved. The examples demonstrate the utility of armFISH in defining chromosomal rearrangements of human cancers.

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CarbocyaninesChromosome AberrationsChromosome PaintingFluorescent DyesHumansIn Situ Hybridization, FluorescenceReagent Kits, DiagnosticSensitivity and SpecificityJournal Article

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R Karhu, M Ahlstedt-Soini, M Bittner, P Meltzer, J M Trent, J J Isola. 2001. Chromosome arm-specific multicolor FISH.. https://doi.org/10.1002/1098-2264(2000)9999%3A9999%3C%3A%3Aaid-gcc1068%3E3.0.co%3B2-9

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