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Glucose-dependent insulin release from genetically engineered K cells.

A T Cheung·B Dayanandan·J T Lewis·G S Korbutt·R V Rajotte·M Bryer-Ash·M O Boylan·M M Wolfe·T J Kieffer
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Abstract

Genetic engineering of non-beta cells to release insulin upon feeding could be a therapeutic modality for patients with diabetes. A tumor-derived K-cell line was induced to produce human insulin by providing the cells with the human insulin gene linked to the 5'-regulatory region of the gene encoding glucose-dependent insulinotropic polypeptide (GIP). Mice expressing this transgene produced human insulin specifically in gut K cells. This insulin protected the mice from developing diabetes and maintained glucose tolerance after destruction of the native insulin-producing beta cells.

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AnimalsBlood GlucoseCell LineCloning, MolecularDiabetes Mellitus, ExperimentalEnteroendocrine CellsGastric Inhibitory PolypeptideGene ExpressionGenetic EngineeringGenetic TherapyGlucoseGlucose Tolerance TestHumansInsulinMiceMice, TransgenicProinsulinPromoter Regions, GeneticProtein PrecursorsStem CellsStreptozocinTransfectionTransgenesTumor Cells, CulturedJournal ArticleResearch Support, Non-U.S. Gov't

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A T Cheung, B Dayanandan, J T Lewis, G S Korbutt, R V Rajotte, M Bryer-Ash, M O Boylan, M M Wolfe, T J Kieffer. 2000-12-08. Glucose-dependent insulin release from genetically engineered K cells.. https://doi.org/10.1126/science.290.5498.1959

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