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PubMed · 1112792

Egasyn, a protein complexed with microsomal beta-glucuronidase.

S Tominco·K Paigen
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Abstract

Two polypeptide chains are present in murine beta-glucuronidase precipitated with a specific anti-beta-glucuronidase antibody F(ab)2 fragment. One is the catalytic subunit of beta-glucuronidase and the other has the properties predicted for the hypothetical beta-gluronidase membrane anchor protein. The new protein, named egasyn, is associated with microsomal, but no lysosomal beta-glucuronidase. It is released from the microsomal beta-glucuronidase complex by heat treatment. The YBR strain of mice carrying the Eg degrees mutation does not form an egasyn-beta-glucuronidase complex and is unable to retain beta-glucuronidase on microsomal membranes.

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AnimalsCarboxylic Ester HydrolasesElectrophoresis, Polyacrylamide GelGlucuronidaseGoatsImmunoglobulin Fab FragmentsIsoenzymesLysosomesMacromolecular SubstancesMembrane GlycoproteinsMiceMice, Inbred StrainsMicrosomes, LiverMolecular WeightMutationPrecipitin TestsProtein BindingProteinsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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S Tominco, K Paigen. 1975-02-10. Egasyn, a protein complexed with microsomal beta-glucuronidase.. https://pubmed.ncbi.nlm.nih.gov/1112792/

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