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DNA sequencing with transposons.

S E Cawley·T P Speed
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Abstract

The use of transposons offers the possibility of a directed approach to DNA sequencing, where a target DNA up to about 6kb in length can be sequenced quickly and with minimal redundancy. Transposons are mobile DNA elements which can be inserted in a reasonably random fashion into the target DNA. An important part of this process is the location of the transposon insertions (known as mapping) and the selection of a sensible subset of transposons to use as priming sites for sequencing reactions. This paper presents a probabilistic method of scoring selected subsets of transposons and a graph-theoretic algorithm for selection of a subset of maximal score.

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AlgorithmsDNA Transposable ElementsModels, StatisticalSequence Analysis, DNAComparative StudyJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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S E Cawley, T P Speed. 2000. DNA sequencing with transposons.. https://doi.org/10.1089/106652701446161

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