Human L1 retrotransposition: cis preference versus trans complementation.

W Wei; N Gilbert; S L Ooi; J F Lawler; E M Ostertag; H H Kazazian; J D Boeke; J V Moran

doi:10.1128/mcb.21.4.1429-1439.2001

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Human L1 retrotransposition: cis preference versus trans complementation.

W Wei·N Gilbert·S L Ooi·J F Lawler·E M Ostertag·H H Kazazian·J D Boeke·J V Moran

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Abstract

Long interspersed nuclear elements (LINEs or L1s) comprise approximately 17% of human DNA; however, only about 60 of the approximately 400,000 L1s are mobile. Using a retrotransposition assay in cultured human cells, we demonstrate that L1-encoded proteins predominantly mobilize the RNA that encodes them. At much lower levels, L1-encoded proteins can act in trans to promote retrotransposition of mutant L1s and other cellular mRNAs, creating processed pseudogenes. Mutant L1 RNAs are mobilized at 0.2 to 0.9% of the retrotransposition frequency of wild-type L1s, whereas cellular RNAs are mobilized at much lower frequencies (ca. 0.01 to 0.05% of wild-type levels). Thus, we conclude that L1-encoded proteins demonstrate a profound cis preference for their encoding RNA. This mechanism could enable L1 to remain retrotransposition competent in the presence of the overwhelming number of nonfunctional L1s present in human DNA.

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Base Sequence DNA Primers Evolution, Molecular Genetic Complementation Test HeLa Cells Humans Long Interspersed Nucleotide Elements Models, Genetic Mutation Open Reading Frames Pseudogenes RNA Processing, Post-Transcriptional RNA, Messenger Recombination, Genetic Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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W Wei, N Gilbert, S L Ooi, J F Lawler, E M Ostertag, H H Kazazian, J D Boeke, J V Moran. 2001. Human L1 retrotransposition: cis preference versus trans complementation.. https://doi.org/10.1128/mcb.21.4.1429-1439.2001

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