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A model for proto-tRNA loading.

K Yusim·S Nir·N Lahav
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Abstract

A kinetic model for loading of proto-tRNA is presented. The kinetic parameters were first estimated from the results of Francklyn & Schimmel (1989;Nature337, 478--481), who studied the aminoacylation of both tRNA and its minihelix. Then these parameters were reduced several-fold, as is more appropriate for the prebiotic world. The simulations revealed a very slow time course of the loading reaction. We also consider a possibility for the proto-tRNA loading without a catalyst and discuss the feasibility of such processes. Analytical approximations are presented for the kinetics of proto-tRNA loading with and without enzyme. An estimate is given for the time required for the development of template- and sequence-directed systems.

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AnimalsComputer SimulationEvolution, MolecularModels, GeneticRNA, TransferJournal Article

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K Yusim, S Nir, N Lahav. 2001-01-07. A model for proto-tRNA loading.. https://doi.org/10.1006/jtbi.2000.2204

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