PubMed · 11190215
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Soulsby. 2000. Mighty microbes!. https://pubmed.ncbi.nlm.nih.gov/11190215/
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The objective of this work was to determine whether persistence of certain Salmonella isolates in fish feed factories involved enhanced resistance to disinfectants or air-drying. Salmonella isolates known to be persistent in fish feed factories and Salmonella isolates from other origins were tested for their sensitivity to nine disinfectants by using a suspension test. More than 5log(10)reduction in viable count for all isolates was only achieved by two of the disinfectants at 80% of the lowest recommended user concentration. However, Salmonella isolates from fish feed factories were not more resistant to disinfectants compared to Salmonella from other origins. For four of the disinfectants, presence of fish feed had a more adverse effect on disinfection efficacy compared to the protein bovine serum albumin (BSA). In general, the Salmonella isolates were more resistant to disinfection than Escherichia coli DSM 682, a strain recommended in testing of disinfectants. The Salmonella isolates were also tested for their ability to survive air-drying at stainless steel surfaces, but there were no differences in survival of isolates from fish feed factories compared to isolates of other origin. In conclusion the Salmonella isolates from fish feed factories were not particularly resistant to disinfection or air-drying at surfaces. The data show that disinfectants to be used against Salmonella should be thoroughly tested and selected, since not all disinfectants appear to be effective against Salmonella.
Two separate studies were conducted to evaluate the utility of glyphosate tolerant canola (GTC) as a feed ingredient in diets fed to rainbow trout. In the first study, two forms of GTC were compared to a parental line, Westar. In the second study, one line of GTC was reevaluated to Westar. In each study, processed canola meals were incorporated at 5, 10, 15, or 20% of the dry diet and a diet containing no canola was fed for comparison. All diets were fed to triplicate groups of fish in each study. In the first study, weight gain, feed efficiency (FE), protein efficiency ratio (PER), and protein retention (PR) were not significantly different in fish fed either Westar or GT200 at any level of substitution. Fish fed GT73 exhibited a gradual reduction in weight gain, FE, and PER as the level of GTC increased. However, the only significant reduction was in weight gain of fish fed 20% GT73 as compared to fish fed 5% GT73. Because of an error in preparing samples prior to the experiment, samples GT200 and GT73 were essentially equivalent in composition. The differences were explained by differences in processing temperatures that occurred after the sample mixing error occurred. In the second study, mean weight gain, PR, and survival were not significantly different among forms of canola. FE and PER values were significantly lower in fish fed 15% Westar as compared to fish fed 10% Westar; other FE and PER values were not significantly different. On the basis of these results, GTC processed into a toasted meal and incorporated into diets for rainbow trout is equivalent to a parental line of canola.
Dihydroergosine (DHES) is the principal toxic alkaloid produced by sorghum ergot (Claviceps africana). It has recently been shown that DHES levels as low as 1 mg/kg in animal feed can cause significant production losses. Quantitative immunoassays for detecting the related rye ergot alkaloid, ergotamine, are described in the literature, but those assays are relatively insensitive for DHES. This paper describes competitive enzyme-linked immunosorbent assays (ELISA) for measuring the DHES concentration in grains and mixed animal feed. The assays were developed using a DHES specific mouse monoclonal antibody and rabbit polyclonal antibodies raised against DHES conjugated to bovine serum albumin. Recoveries of between 77 and 103% were obtained from spiked grain using a simple, one step extraction with 70% methanol. Both the monoclonal and the polyclonal assays are capable of detecting DHES concentrations above 0.01 mg/kg, but quantification is most reliable at concentrations of 0.1 mg/kg or higher.