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PubMed · 11201575

Comparison among three methods for mycobacteria identification.

M Mondragón-Barreto·C A Vázquez-Chacón·C Barrón-Rivero·P Acosta-Blanco·K C Jost·S Balandrano·H Olivera-Díaz
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Abstract

OBJECTIVE: To compare three methods: Biochemical tests, high-performance liquid chromatography (HPLC) and polymerase chain reaction-restriction fragments length polymorphism (PCR-RFLP), for the identification of mycobacteria, and to perform a cost-benefit analysis to define an optimum identification algorithm. MATERIAL AND METHODS: One-hundred-and-seven mycobacteria isolates were identified by the three methods at Instituto de Diagnóstico y Referencia Epidemiológicos, between February of 1999 and January of 2000 and the results were compared with those of a reference laboratory using the Q-Cochran statistical test. RESULTS: PCR-RFLP was the most rapid and specific procedure but also the most expensive; biochemical tests excelled for identification of Mycobacterium tuberculosis, but were lengthy and expensive for other mycobacteria; HPLC ranked in the middle for price, speed and specificity. CONCLUSIONS: Considering the expected proportion of M. tuberculosis, the following algorithm was proposed: Initially, biochemical tests should be performed; if the results indicate a non-tuberculous mycobacteria, the isolate should be analyzed with HPLC; if results are unclear, the isolate should be analyzed using PCR-RFLP. Isolates showing a previously undescribed PCR-RFLP pattern should be characterized by DNA sequencing.

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Bacterial ProteinsBacterial Typing TechniquesCell WallChaperonin 60ChaperoninsChromatography, High Pressure LiquidCosts and Cost AnalysisDNA, BacterialDeoxyribonucleases, Type II Site-SpecificDouble-Blind MethodHumansMycobacteriumMycobacterium InfectionsMycobacterium avium ComplexMycobacterium tuberculosisMycolic AcidsNontuberculous MycobacteriaPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthSensitivity and SpecificitySpecies SpecificityTime FactorsComparative StudyJournal Article

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M Mondragón-Barreto, C A Vázquez-Chacón, C Barrón-Rivero, P Acosta-Blanco, K C Jost, S Balandrano, H Olivera-Díaz. Comparison among three methods for mycobacteria identification.. https://doi.org/10.1590/s0036-36342000000600003

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