PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 11202229

Recent developments in flow cytometry.

A Scheffold·F Kern
View original source ↗Export metadata ↓

Abstract

Flow cytometry has experienced considerable expansion of available parameters during the last years. Various new techniques that allow the direct analysis of antigen-specific lymphocytes by direct labeling with antigen or according to functional parameters following in vitro antigen stimulation will have enormous impact on immunological research, diagnosis, and therapy. This article concentrates on the basic principles and applications of these important technological developments in flow cytometry and focuses on the analysis of specific lymphocytes.

Explore related subjects

CytokinesEpitopes, B-LymphocyteEpitopes, T-LymphocyteFlow CytometryFluorescent Antibody TechniqueHLA AntigensHumansImmunophenotypingLymphocyte ActivationSensitivity and SpecificityT-LymphocytesJournal ArticleReview

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

A Scheffold, F Kern. 2000. Recent developments in flow cytometry.. https://doi.org/10.1023/a%3A1026454225515

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2003

Acquired T-helper 1 lymphocyte anergy following infection with Mycobacterium ulcerans.

We describe a patient with acquired T-helper lymphocyte anergy to mycobacteria following infection with Mycobacterium ulcerans. Before infection, the patient's peripheral blood mononuclear cells responded to in vitro stimulation with M. ulcerans by producing Th1 cytokines, but, after she developed an ulcer, the response was shifted toward production of Th2 cytokines. Immunomodulatory therapy may be an effective intervention for Buruli ulcer.

Cytokines↗
PubMed2003

Map kinase signaling pathways and hematologic malignancies.

Mitogen-activated protein (Map) kinases are widely expressed serine-threonine kinases that mediate important regulatory signals in the cell. Three major groups of Map kinases exist: the p38 Map kinase family, the extracellular signal-regulated kinase (Erk) family, and the c-Jun NH2-terminal kinase (JNK) family. The members of the different Map kinase groups participate in the generation of various cellular responses, including gene transcription, induction of cell death or maintenance of cell survival, malignant transformation, and regulation of cell-cycle progression. Depending on the specific family isoform involved and the cellular context, Map kinase pathways can mediate signals that either promote or suppress the growth of malignant hematopoietic cells. Over the last few years, extensive work by several groups has established that Map kinase pathways play critical roles in the pathogenesis of various hematologic malignancies, providing new molecular targets for future therapeutic approaches. In this review, the involvement of various Map kinase pathways in the pathophysiology of hematologic malignances is summarized and the clinical implications of the recent advances in the field are discussed.

Cytokines↗
PubMed2003

Breast milk fatty acids, eicosanoids, and cytokines in mothers with and without allergic disease.

Allergic disease (AD), including atopic eczema, asthma, allergic rhinitis, and food allergy, is characterized by an imbalance between cytokines produced by distinct T-helper cell subtypes. Whether this imbalance can be transferred from mother to breast milk remains to be established. The objective was to investigate the concentrations and interactions of nutritional and inflammatory factors in breast milk. Breast milk samples were collected from mothers with AD (n = 43) and without AD (n = 51). The concentrations of transforming growth factor (TGF)-beta2, tumor necrosis factor-alpha, IL-4, IL-10, prostaglandin E2, and cysteinyl leukotrienes were measured by immunoassays and fatty acid composition by gas chromatography. Mothers with AD had a lower concentration of TGF-beta2 in breast milk [median (interquartile range), 420 (278-701) ng/L] compared with those without AD [539 (378-1108) ng/L; p = 0.003], whereas other cytokines, prostaglandin E2, and cysteinyl leukotriene concentrations or fatty acid composition were not significantly different between the groups. The breast milk inflammatory factors and fatty acid composition were shown to be related. A positive association was observed between TGF-beta2 and the proportion of polyunsaturated fatty acids (p = 0.038) and a negative association between TGF-beta2 and the proportion of saturated fatty acids (p = 0.029) in breast milk. The reduced TGF-beta2 concentration in the breast milk of mothers with AD may interfere with the development of the mucosal immune system of the breast-fed infant. The observed associations between nutritional and inflammatory factors in breast milk suggest that it may be possible to influence the immunologic properties of breast milk by dietary intervention of the mother.

Cytokines↗