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Nano-electrospray ionization mass spectrometry: addressing analytical problems beyond routine.

M Karas·U Bahr·T Dülcks
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Abstract

The advent of nano-electrospray ionization (nano-ESI) has considerably extended the usability of ESI in the analytical mass spectrometric laboratory. One of the remarkable features of nano-ESI is its extremely low sample consumption. Only a few microliters of analyte solution (10(-5)-10(-8) M) are sufficient for molecular weight determination and structural investigations by MS/MS. But nano-ESI is more than just a minimized-flow ESI; the low solvent flow rate also affects the mechanism of ion formation. As a consequence, the area of ESI-MS applications is significantly enhanced. Oligosaccharides, glycosides as well as glycoproteins can be analyzed more easily than with normal ion spray. The same holds for the analysis of non-covalent complexes sprayed directly from aqueous solutions.

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AnimalsGlycoproteinsGlycosidesHumansMicrochemistryMiniaturizationOligosaccharidesSample SizeSolventsSpectrometry, Mass, Electrospray IonizationJournal ArticleReview

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M Karas, U Bahr, T Dülcks. Nano-electrospray ionization mass spectrometry: addressing analytical problems beyond routine.. https://doi.org/10.1007/s002160051561

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