Multiplex screening for functionally rearranged immunoglobulin variable regions reveals expression of hybridoma-specific aberrant V-genes.

G Vidarsson; J G van de Winkel; M A van Dijk

doi:10.1016/s0022-1759(00)00337-9

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Multiplex screening for functionally rearranged immunoglobulin variable regions reveals expression of hybridoma-specific aberrant V-genes.

G Vidarsson·J G van de Winkel·M A van Dijk

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Abstract

Modification of antibody effector functions is commonly performed by chimerization or humanization. Cloning of antibody variable regions from hybridomas represents a first step that is frequently hampered by the expression of non-functionally rearranged variable regions in hybridoma cells that originate from MOPC21-derived fusion partners. We now present a simple method to clone functionally rearranged V-genes, based on V-gene-specific multiplex PCR screening. Using this method we document the expression of aberrant V-genes that originate from the original B-cell used for the hybridoma generation, not from the fusion partner, and are - thus - hybridoma specific.

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Animals Base Sequence Gene Rearrangement, B-Lymphocyte Genes, Immunoglobulin Hybridomas Immunoglobulin Variable Region Immunologic Techniques Mice Molecular Sequence Data Journal Article Research Support, Non-U.S. Gov't

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G Vidarsson, J G van de Winkel, M A van Dijk. 2001-03-01. Multiplex screening for functionally rearranged immunoglobulin variable regions reveals expression of hybridoma-specific aberrant V-genes.. https://doi.org/10.1016/s0022-1759(00)00337-9

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