Advances with supercritical fluids.

W H Hauthal

doi:10.1016/s0045-6535(00)00332-5

Home/Archive/PubMed

PubMed · 11233819

Advances with supercritical fluids.

W H Hauthal

View original source ↗Export metadata ↓

Abstract

In the last decade, supercritical fluids more and more have been proved as environmentally benign media for chemical and related processes. Many new processes and products have been developed, using the inherent physical and chemical properties of supercritical fluids. Moreover, these processes also promise economic effects. The prerequisites for this success however, are a sound knowledge of physico-chemical properties of--and phenomena in--supercritical mixtures and the availability of other chemical engineering data. This requires an effective exchange of knowledge between a large number of branches of science. In the following, a lot of recent papers will be cited, which should give an overview of actual results on fundamentals and their applications.

Explore related subjects

Chemical Engineering Chemical Phenomena Chemistry, Physical Conservation of Natural Resources Environmental Pollution Manufactured Materials Models, Chemical Solvents Journal Article Research Support, Non-U.S. Gov't Review

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

W H Hauthal. 2001. Advances with supercritical fluids.. https://doi.org/10.1016/s0045-6535(00)00332-5

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

Trapping transient protein-protein interactions in polyketide biosynthesis.

Transient biomolecular interactions are essential for biological processes, but strategies for studying them have remained elusive. A paper in this issue shows how natural enzymatic activities can be exploited to examine protein-protein interactions in fatty acid synthase.

Chemical Engineering↗

PubMed2006

Existing and emerging detection technologies for DNA (Deoxyribonucleic Acid) finger printing, sequencing, bio- and analytical chips: a multidisciplinary development unifying molecular biology, chemical and electronics engineering.

The current status and research trends of detection techniques for DNA-based analysis such as DNA finger printing, sequencing, biochips and allied fields are examined. An overview of main detectors is presented vis-à-vis these DNA operations. The biochip method is explained, the role of micro- and nanoelectronic technologies in biochip realization is highlighted, various optical and electrical detection principles employed in biochips are indicated, and the operational mechanisms of these detection devices are described. Although a diversity of biochips for diagnostic and therapeutic applications has been demonstrated in research laboratories worldwide, only some of these chips have entered the clinical market, and more chips are awaiting commercialization. The necessity of tagging is eliminated in refractive-index change based devices, but the basic flaw of indirect nature of most detection methodologies can only be overcome by generic and/or reagentless DNA sensors such as the conductance-based approach and the DNA-single electron transistor (DNA-SET) structure. Devices of the electrical detection-based category are expected to pave the pathway for the next-generation DNA chips. The review provides a comprehensive coverage of the detection technologies for DNA finger printing, sequencing and related techniques, encompassing a variety of methods from the primitive art to the state-of-the-art scenario as well as promising methods for the future.

Chemical Engineering↗

PubMed2006

Engineering of protein thermodynamic, kinetic, and colloidal stability: Chemical Glycosylation with monofunctionally activated glycans.

In this work we establish the relationship between chemical glycosylation and protein thermodynamic, kinetic, and colloidal stability. While there have been reports in the literature that chemical glycosylation modulates protein stability, mechanistic details still remain uncertain. To address this issue, we designed and coupled monofunctional activated glycans (lactose and dextran) to the model protein alpha-chymotrypsin (alpha-CT). This resulted in a series of glycoconjugates with variations in the glycan size and degree of glycosylation. Thermodynamic unfolding, thermal inactivation, and temperature-induced aggregation experiments revealed that chemical glycosylation increased protein thermodynamic (Delta G(25 degrees C)), kinetic (t(1/2)(45 degrees C)), and colloidal stability. These results highlight the potential of chemical glycosylation with monofunctional activated glycans as a technology for increasing the long-term stability of liquid protein formulations for industrial and biotherapeutic applications.

Chemical Engineering↗