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Intralymphatic immunization enhances DNA vaccination.

K J Maloy·I Erdmann·V Basch·S Sierro·T A Kramps·R M Zinkernagel·S Oehen·T M Kündig
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Abstract

Although DNA vaccines have been shown to elicit potent immune responses in animal models, initial clinical trials in humans have been disappointing, highlighting a need to optimize their immunogenicity. Naked DNA vaccines are usually administered either i.m. or intradermally. The current study shows that immunization with naked DNA by direct injection into a peripheral lymph node enhances immunogenicity by 100- to 1,000-fold, inducing strong and biologically relevant CD8(+) cytotoxic T lymphocyte responses. Because injection directly into a lymph node is a rapid and easy procedure in humans, these results have important clinical implications for DNA vaccination.

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AnimalsAntigens, ViralCell LineDNA, ViralEpitopes, T-LymphocyteGlycoproteinsHomeodomain ProteinsImmunodominant EpitopesInjections, IntralymphaticLymphocytic ChoriomeningitisLymphocytic choriomeningitis virusMiceMice, Inbred C57BLMice, KnockoutPeptide FragmentsPlasmidsT-Lymphocytes, CytotoxicVaccinationVaccines, DNAViral ProteinsViral VaccinesJournal ArticleResearch Support, Non-U.S. Gov't

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K J Maloy, I Erdmann, V Basch, S Sierro, T A Kramps, R M Zinkernagel, S Oehen, T M Kündig. 2001-02-27. Intralymphatic immunization enhances DNA vaccination.. https://doi.org/10.1073/pnas.051630798

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