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Gene expression profiling of malignant mesothelioma cell lines: cDNA array study.

E Kettunen·A M Nissén·T Ollikainen·M Taavitsainen·J Tapper·K Mattson·K Linnainmaa·S Knuutila·W El-Rifai
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Abstract

To reveal genes relevant for malignant mesothelioma (MM), we carried out cDNA array experiments on 4 MM cell lines and 2 primary mesothelial cell cultures established from pleural fluid of non-cancer patients. Human cancer gene filters including 588 genes were used for the cDNA array experiments. Our study revealed 26 over-expressed genes that play a role in the regulation of cell fate, cell cycle, cell growth and DNA damage repair and 13 under-expressed genes encoding growth factors, receptors and proteins involved in cell adhesion, motility and invasion to be common to 3 or 4 MM cell lines. We confirmed the cDNA array results using RT-PCR for 5 of the over-expressed genes and for 3 of the under-expressed genes. Our study presents gene expression profiles in MM cell lines and shows the involvement of several genes, such as those encoding JAGGED1, ser/thr protein kinase NIK, Ku80 and cyclin D2, novel in MM.

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DNA, ComplementaryDown-RegulationElectrophoresis, Agar GelEpitheliumGene Expression Regulation, NeoplasticHumansMesotheliomaNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionTumor Cells, CulturedUp-RegulationJournal ArticleResearch Support, Non-U.S. Gov't

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E Kettunen, A M Nissén, T Ollikainen, M Taavitsainen, J Tapper, K Mattson, K Linnainmaa, S Knuutila, W El-Rifai. 2001-02-15. Gene expression profiling of malignant mesothelioma cell lines: cDNA array study.. https://doi.org/10.1002/1097-0215(200002)9999%3A9999%3C%3A%3Aaid-ijc1094%3E3.0.co%3B2-m

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