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PubMed · 11282341

Novel methods for biocatalyst screening.

D Wahler·J L Reymond
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Abstract

There have been a number of recent advances in catalysis assays applicable for screening biocatalyst libraries in high-throughput format. These include instrumental assays such as high-performance liquid chromatography, mass spectrometry, capillary electrophoresis and IR-thermography, reagent-based assays producing spectroscopic signals (UV/VIS or fluorescence) in response to reaction progress, and assays based on fluorogenic or chromogenic substrates. These fluorogenic substrates enable the assaying of a variety of enzymes in enantioselective and stereoselective manner, including alcohol dehydrogenases, aldolases, lipases, amidases, epoxide hydrolases and phosphatases.

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Carrier ProteinsCatalysisChromatography, High Pressure LiquidChromogenic CompoundsCombinatorial Chemistry TechniquesDrug Evaluation, PreclinicalElectrophoresis, CapillaryEnzyme-Linked Immunosorbent AssayEnzymesFluorometryGene LibraryMass SpectrometrySelection, GeneticSubstrate SpecificityJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Review

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D Wahler, J L Reymond. 2001. Novel methods for biocatalyst screening.. https://doi.org/10.1016/s1367-5931(00)00184-8

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