SMRT as a T3SF-binding protein.

K Onishi; M V Kato; F Bai; T Sakai

doi:10.3892/ijo.18.5.985

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PubMed · 11295045

SMRT as a T3SF-binding protein.

K Onishi·M V Kato·F Bai·T Sakai

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Abstract

p53-binding consensus-like sequence (T3SF) is located in the murine promoter region of tissue inhibitor of metalloproteinase 3 gene. To identify the genes that encode proteins that bind to T3SF DNA sequence, we screened a cDNA library using the Southwestern technique. The SMRT gene was cloned as one of the candidates. Addition of antibody against SMRT reduced the intensity of a band that is supposed to contain SMRT in electrophoresis mobility shift assay, although antibody against p53 had no effect. Ultraviolet (UV)-irradiation reduced the intensity of the SMRT complex whereas p53 complex was stabilized by UV-irradiation. These results suggest that SMRT may bind to T3SF sequence in p53-independent manner and dissociate from the sequence by UV irradiation.

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Binding Sites Blotting, Southern Blotting, Western DNA-Binding Proteins Electrophoresis, Agar Gel Gene Library Humans Nuclear Receptor Co-Repressor 2 Repressor Proteins Tissue Inhibitor of Metalloproteinase-3 Tumor Cells, Cultured Tumor Suppressor Protein p53 Journal Article Research Support, Non-U.S. Gov't

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K Onishi, M V Kato, F Bai, T Sakai. 2001. SMRT as a T3SF-binding protein.. https://doi.org/10.3892/ijo.18.5.985

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