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PubMed · 11325745

Chemical force microscopy with active enzymes.

M Fiorini·R McKendry·M A Cooper·T Rayment·C Abell
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Abstract

The adhesion forces have been measured between an atomic force microscope tip derivatized with an active enzyme, shikimate kinase, and an ATP mimic immobilized on a gold surface. Experiments with competitive binding of other ligands in solution show that the observed adhesion forces arise predominantly from specific interactions between the immobilized enzyme and surface-bound adenine derivative. These experiments represent a step in the development of a screening methodology based upon chemical force microscopy.

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Binding, CompetitiveCatalysisCell AdhesionEnzymesGoldKineticsLigandsMicroscopy, Atomic ForcePhosphotransferases (Alcohol Group Acceptor)Protein BindingShikimic AcidSpectrophotometry, InfraredSurface Plasmon ResonanceTime FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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M Fiorini, R McKendry, M A Cooper, T Rayment, C Abell. 2001. Chemical force microscopy with active enzymes.. https://doi.org/10.1016/s0006-3495(01)76215-7

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