Model systems: Studying molecular recognition using bacterial n-hybrid systems.

J C Hu

doi:10.1016/s0966-842x(01)02019-4

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Model systems: Studying molecular recognition using bacterial n-hybrid systems.

J C Hu

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Abstract

Since the first description of the yeast two-hybrid system, related genetic assays for protein-protein interactions have become popular and powerful tools for structure-function analysis on the scale of individual proteins or whole proteomes. After a somewhat surprising lag, similar systems have recently been described for use in bacterial hosts. n-hybrid modifications of the original yeast system have been used to examine interactions with DNA, RNA and small molecules, and other modifications have improved throughput for genomic applications. Bacterial n-hybrid systems are being designed for a similar array of uses. Will the bacterial systems be as popular as the yeast n-hybrid systems? Only time will tell.

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Bacteria Bacterial Proteins Genome, Bacterial Two-Hybrid System Techniques Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Review

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J C Hu. 2001. Model systems: Studying molecular recognition using bacterial n-hybrid systems.. https://doi.org/10.1016/s0966-842x(01)02019-4

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